| Assay Method Information | |
| | TRPA1 Inhibitor Activity Assay |
| Description: | The cells were bathed in an extracellular solution containing 80 mM NaCl, 60 mM NMDG, 4 mM KCl, 2 mM CaCl2), 6 mM MgCl2, 5 mM Glucose, 10 mM HEPES, 3 mM HEDTA; pH adjusted to 7.4 with NaOH; 305-310 mOsm. All compounds were dissolved in DMSO at 30 mM. The internal solution contained 10 mM CsCl, 110 mM CsF, 10 mM NaCl, 10 mM EGTA, 10 mM HEPES, 4 mM MgATP, 0.25 mM NaGTP, 4 mM BAPTA; pH adjusted to 7.2 with CsOH; 285-290 mOsm. Compound stock solutions were freshly diluted with external solution to concentrations of 3 nM, 10 nM 30 nM, 100 nM, 300 nM, 1 μM, 3 μM, 10 μM, and 30 μM. The highest content of DMSO (0.1%) was present at 30 μM. |
| Affinity data for this assay | |
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