| Assay Method Information | |
| | Kinase Inhibition Assay |
| Description: | Kinase LRRK2 G2019S protein was derived from Carna, LRRKtide was derived from Signalchem, ATP was derived from Promega, and DMSO was derived from Sigma. All assays were performed at room temperature. The test compounds were dissolved and diluted, and then added to a 384-well plate. 2.5 μL of 2× kinase reaction buffer was added, and the mixture was centrifuged. 2.5 μL of a mixture of 2× substrate and ATP was added, and the mixture was centrifuged. 4 μL of ADP-Glo reagent was added, and the mixture was incubated at room temperature for 40 min. 8 μL of LRRKtide was added, and the mixture was incubated at room temperature for 40 min. Then, the data were read using the Envision 2104 multi-label Reader. |
| Affinity data for this assay | |
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