| Assay Method Information | |
| | Human SMOX Assay |
| Description: | Test compounds (75 nL) dissolved in DMSO were added to a 384-well white assay plate (Greiner Bio-one, catalog #784075) using an ECHO Acoustic Dispenser (Labcyte). Each compound was serially diluted 3-fold in DMSO to prepare an 11-point dose response curve (columns 1-22). Column 23 and 24 contained 75 nL of 2 mM chlorhexidine and DMSO, respectively, to represent the 100% inhibited versus the neutral control reactions. |
| Affinity data for this assay | |
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