| Assay Method Information | |
| | Methods of ELISA Assay on Screening Lp(a) Assembly Inhibition |
| Description: | 1) Added 100 μL of capture antibody to each well of a pre-coated ELISA plate and incubated overnight at 25° C.2) Added 300 μL of washing solution to each well and washed 5 times for 1 min each time.3) Added 100 μL of blocking solution to each well and incubated at 25° C. for 2 h.4) Added 300 μL of washing solution to each well and washed 5 times for 1 min each time.5) Added 100 μL of sample to each well and incubated at 25° C. for 1 h.6) Added 300 μL of washing solution to each well and washed 5 times for 1 min each time.7) Added 100 μL of detection antibody to each well and incubated at 25° C. for 1 h.8) Added 300 μL of washing solution to each well and washed 5 times for 1 min each time.9) Added 100 μL substrate solution (Color Reagent A: Color Reagent B, 1:1) to each well and incubated for 20 min at 25° C. in the dark.10) Added 50 mL of stop solution to each well, gently blowed and sucked several times with the pipette tip, and read within 5 min. |
| Affinity data for this assay | |
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