| Assay Method Information | |
| | TYK2 (TYK2/JAK2 PSTAT4 T-BLAST) Alpha Screen Assay |
| Description: | IL-12 is known to transduce its signal through IL-12 receptor via Jak2 and/or Tyk2. For that reason, the activity against Tyk2/Jak2 was measured by determining the inhibition of IL-12 induced phosphorylation of STAT4 in T-blast cells. From these tests, the EC50 (effective concentration for 50% maximal response) values were determined in μM for the test compounds. If the compounds showed minimal activity in the Jak2 (PTAT5 UT7) alpha screen assay (e.g., >25 μM activity), then it was stipulated that the activity from the Tyk2 (Tyk2/Jak2 T-Blast) alpha screen assay is driven by inhibition of Tyk2. See, e.g., Sohn et al., J. Immunology (2013) 2205-2216. |
| Affinity data for this assay | |
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