| Assay Method Information | |
| | Z′-LYTE Assay |
| Description: | Using a bar-coded Corning, low volume NBS, black 384-well plate (Corning Cat. #4514), 100 nL of 100× test compound in 100% DMSO was added. Followed by 2.4 μL of kinase buffer, 5 μL of 2× peptide/kinase mixture, and 2.5 μL-4×ATP solution. The plate was then shaken for 30 seconds and held at room temperature for a 60 minute kinase reaction incubation period. At which point 5 μL of development reagent solution was added and the plate was shaken for 30 seconds. The plate was held again at room temperature for a 60 minute development reaction incubation period and then read on a fluorescence plate reader and analyzed. |
| Affinity data for this assay | |
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