| Assay Method Information | |
| | PBB5 competition assay |
| Description: | PBB5 competition assay: 2 μM of α-synuclein was incubated with 0.26 μM 2-(4-(2-(methylamino)pyridine-5-yl)-1,3-butadiene-1-yl)benzothiazole-6-ol (PBB5) and serially diluted compound (three-fold serial dilution, from 10 to 0.001 μM) in a 96-well plate at 37° C. for 1 hour. Fluorescence intensity (excitation/emission=530/690 nm) of PBB5 was read by microplate spectrometer. Percentage of competition was calculated using the following equation:(Max−well of compound alone)/(Max−Min)*100%, where Max=(α-synuclein+PBB5) signals−(buffer+PBB5) signals; and Min=(buffer+PBB5) signals−(buffer+PBB5) signals. |
| Affinity data for this assay | |
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