| Assay Method Information | |
| | SGLT1 Glucose Transport Test |
| Description: | 1) Experimental buffer: 10 mM 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES), 1.2 mM magnesium chloride (MgCl2), 4.7 mM potassium chloride (KCl), 2.2 mM calcium chloride (CaCl2) and 120 mM sodium chloride (NaCl).2) The compound was diluted with 100% dimethyl sulfoxide (DMSO) with 1 mM as the starting concentration and 8 points of 5-fold serial dilutions.3) 3 μM [14C]-labeled methyl α-D-glucopyranoside was prepared with experimental buffer.4) The cells were treated with 49 μL of experimental buffer, 1 μL of the compound which was gradient diluted, and 50 μL of 3 μM [14C] isotope-labeled sugar solution at 37° C. for 2 hours.5) The isotope detector (Micro beta Reader) was used to read.6) The data was calculated by GraphPad Prism 5.0 software: log(inhibitor) vs. response—Variable slope to obtain the IC50 value of the test compound |
| Affinity data for this assay | |
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