| Assay Method Information | |
| | DNA-PK Biochemical Assay Using Reaction Biology's HotSpot Kinase Assay Protocol |
| Description: | Reagent: Base Reaction buffer; 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.01% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, 1% DMSO Required cofactors are added individually to each kinase reaction.Reaction Procedure: 1. Prepare substrate in freshly prepared Reaction Buffer; 2. Deliver any required cofactors to the substrate solution above; 3. Deliver kinase into the substrate solution and gently mix; 4. Deliver compounds in 100% DMSO into the kinase reaction mixture by Acoustic technology (Echo550; nanoliter range), incubate for 20 min at room temperature; 5. Deliver 33P-ATP into the reaction mixture to initiate the reaction; 6. Incubate for 2 hours at room temperature; 7. Detect kinase activity by P81 filter-binding method. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |