| Assay Method Information | |
| | Surface Plasmon Resonance Experiment |
| Description: | Experimental method: Surface plasmon resonance test was carried out on BIACORE T200 instrument (GE Company). Fresh purified grade Src kinase SH3 domain protein (concentration 2 mg/ml) was diluted to 0.1 mg/ml with 10 mM CH3COONa (pH 4.2) and then SH3 protein was coupled to the CM5 chip by standard amino coupling methods. The compounds of the invention were diluted in a gradient using a buffer solution (20 mM Tris-HCl, PH 8.0, 100 mM NaCl) for 60 seconds and dissociated for 120 seconds at a flow rate of 20 μl/s, respectively. The changes in response values over time were recorded and analyzed by the BIA Evaluation Software (GE Healthcare) program to obtain the dissociation constants KD for the different compounds with SH3 protein. |
| Affinity data for this assay | |
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