| Assay Method Information | |
| | Surface Plasmon Spectroscopy |
| Description: | Binding of affinity of SHIM compounds has been determined using surface plasmon spectroscopy on a Biacore T200 (GE Healthcare). Biotinylated PCSK9 (ACRO Biosystems) at 1 μg/mL has been captured on a chip on which streptavidin has been immobilized with amine-directed chemistry according to the manufacturer's instructions (6000 RU) to a density of 6000 RU in PBS-P (potassium buffered saline, 0.005% Tween 20, pH7.4) buffer with 1% DMSO. SHIMs at 10, 3.3, 1.1, 0.33 and 0.11 uM were injected at 30 uL/min. The data were fitted to 1:1 Langmuir binding model using Bioeval 4.3 software (GE Healthcare) to obtain the KD values. Double reference method of analysis was used with the reference channel containing a reference biotinylated protein captured at 6000 RU. |
| Affinity data for this assay | |
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