Assay Method Information | |
| CDK Inhibition Assay |
Description: | In vitro CDK assay using purified enzyme, was incubated at 30 °C with substrate, and test compounds in the presence of ATP/ [gamma-32P] ATP. 32P incorporation was measured with a Top Count (Packard). IC50 values were calculated from dose-response curves. |
Affinity data for this assay | |
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