| Assay Method Information | |
| | Enzyme Assay and Determination of the Inhibition Constants. |
| Description: | The enzyme reactions were initiated by the addition of substrate, and the color developed from the release of p-nitroanilide from each chromogenic substrate was monitored continuously for 5 min at 405 nm on a microtiter plate reader. The initial velocities measured were used to determine the amount of inhibitor required to diminish 50% of the control velocity; this concentration was defined as the IC50 of the inhibitor. The apparent Ki values were then calculated according to the Cheng-Prusoff equation: Ki = IC50/1 + [S]/Km. |
| Affinity data for this assay | |
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