Assay Method Information | |
| Protease Inhibition Assay |
Description: | The Ki values were determined by substrate cleavage assay using fluorogenic substrate, 2-(aminobenzoyl)-Thr-Ile-Nle-Phe(p-NO2)-Gln-Arg-NH2. A standard curve relating changes in fluorescent intensity to changes in concentration of product was used to convert fluorescent changes into molar velocities. The amount of cleavage was determined by HPLC. |
Affinity data for this assay | |
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