| Assay Method Information | |
| | Enzyme Assay and Determination of the Inhibition Constants |
| Description: | Each enzyme was assayed with a set of different concentrations of each inhibitor. After addition of the appropriate substrate, the rate of hydrolysis was measured by monitoring the increase in absorbance at 405 nM for 5 minutes. Ki(apparent) values were calculated from enzyme velocity curves using the software package BatchKi (BioKin Ltd, Madison, WI). These apparent inhibition constants were then converted to Ki values by assuming competitive inhibition and using the formula Ki = Ki(apparent)/(1 + S/Km). |
| Affinity data for this assay | |
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