| Assay Method Information | |
| | PDE Activity Assay |
| Description: | PDE4 enzyme activity was monitored by measuring the hydrolysis of [3H]-cAMP to [3H]-AMP using SPA kit. [3H]-AMP was captured by the SPA beads, and quantified on a scintillation counter. In each assay, substrate concentration was 200 nM, near the Km values for the enzymes under the conditions of the assays. |
| Affinity data for this assay | |
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