| Assay Method Information | |
| | Thermal Denaturation Assay |
| Description: | UV thermal melt experiments were carried out in a quartz cuvette loaded with sample containing enzyme and each test compound using a spectrophotometer. For each measurement, absorbance data at 230 nm was collected as the temperature was scanned from 25 to 80 C at a ramp rate of 0.2 C /min. The melting temperature (Tm) for each sample was calculated as the maximum deflection point of the first derivative of the melting transition. The Tm values are converted into Kd values using the equation: Tm =3.08 (log Ka) + 31.2, where Ka= 1/Kd. |
| Affinity data for this assay | |
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