Assay Method Information | |
| Fluorescence Polarization (FP) Assay |
Description: | The assay is based upon displacement of a fluorescently labeled molecule, which binds specifically to the ATP-binding site of full-length human Hsp90. The competitive displacement was monitored by a decrease in fluorescence polarization of the probe-Hsp90 complex when the inhibitor binds. IC50 values were calculated on the difference in anisotropy from the first and second reads. |
Affinity data for this assay | |
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