| Assay Method Information | |
| | Determination of Inhibition Constants |
| Description: | The measurements were carried out on a microplate reader. Two concentrations of the substrate and five concentrations of the inhibitor were used in the assay. Three minutes after the addition of the enzyme to the reaction solution containing substrate and test compound, acetic acid (50%) was added to quench the reaction, and the optical density was measured at 405 nm. The Ki-values were calculated according to Dixon using a linear regression program. The Ki-values reported are means from at least three determinations. |
| Affinity data for this assay | |
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