| Assay Method Information | |
| | PKB In Vitro Enzyme Assay |
| Description: | The purified PKB beta enzyme was assayed with a peptide substrate and test compound in the presence of 30 uM ATP/ [gamma-33P]ATP in 96-well plates. IC50 is the concentration of inhibitor that inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-33P] labeled ATP to substrate peptide. IC50 values were determined from an eight-point concentration range run in duplicate and calculated in GraphPad Prism 4.00. |
| Affinity data for this assay | |
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