| Assay Method Information | |
| | In Vitro BACE Activity Assay |
| Description: | The assay was performed using a 96-well format on a HPLC equipped with four 96-well plate holders. Test compounds were preincubated with enzymes for 30min, followed by the addition of substrate. The solution was then incubated for an additional 30 min at room temperature. After the reaction was terminated, the solution was filtered, and the filtrate was subjected to HPLC analysis. The substrate and product were monitored by fluorescence detection (340nm excitation, 440 nm emission). |
| Affinity data for this assay | |
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