| Assay Method Information | |
| | FRET-based Peptide Cleavage Assay |
| Description: | A homogenous, continuous fluorescence resonance energy transfer (FRET) assay was used to assess compound inhibition of enzyme based on the cleavage of its substrate. Kinetic rates were calculated and IC50 values were determined by fitting the percent inhibition, as a function of compound concentration, to the Hill equation. |
| Affinity data for this assay | |
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