| Assay Method Information | |
| | PDK1-mediated AKT2 Activation Assay (cAKT2 33P-SPA) |
| Description: | The coupled assay can detect inhibitors of AKT2 activation, as well as direct inhibitors of PDK1 or AKT2. Inactive AKT2 is activated in situ by incubating with PDK1 and PtdIns-3, 4-P2 in the presence of compound, 33P-ATP, and a biotinylated peptide substrate for AKT2. The peptide is captured on streptavidin-coated SPA beads for detection. |
| Affinity data for this assay | |
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