Assay Method Information

Assay Name:  Her-2 Degradation Assay
Description:  EC50 was defined as the concentration of the compound at which there was 50% degradation of the Her-2/neu protein in MCF7 breast carcinoma cells. Samples were analyzed using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA) equipped with an argon-ion laser that emits 488 nm light for excitation of the phycoerythrin fluorochrome. A fluorescence histogram was generated, and the mean fluorescence intensity (MFI) of each sample was determined using Cellquest software.
Affinity data for this assay
 

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