Assay Method Information | |
| Flow Cytometric Assay |
Description: | The compounds were tested to determine their ENT1 nucleoside transporter binding ability by a flow cytometric assay using human leukemia K562 cells incubated with fluorescent probe in the presence or absence of varying concentrations of test compounds. Flow cytometric measurements of cell-associated fluorescence were performed with a FACSCalibur instrument (Becton Dickinson, San Jose, CA). Percentage (%) of control (ENT1 transporter specific fluorescence in the presence of SAENTA-fluorescein without test compounds) was calculated for each sample. The results were fed into the GraphPad PRISM to derive concentration-dependent curves. From these curves, the IC50 values were obtained and used to calculate inhibition constants (Ki). |
Affinity data for this assay | |
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