| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | Progress curves were obtained by initiation of urease reaction with addition of purified enzyme into assay mixtures containing increasing concentrations of inhibitors and urea, which are in the range of 2 to 100 mM. Each assay was run in triplicate. Ki values were dertermined from Lineweaver-Burk plots after testing at least 5 inhibitor concentrations. IC50 values were obtained from measurements performed in the presence of saturating urea concentration 100 mM. |
| Affinity data for this assay | |
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