| Assay Method Information | |
| | Fluorescence Anisoptrophy Kinase Binding Assay |
| Description: | The kinase enzyme, fluorescent ligand and a variable concentration of test compound are incubated together to reach thermodynamic equilibrium under conditions such that in the absence of test compound the fluorescent ligand is significantly (>50%) enzyme bound and in the presence of a sufficient concentration (>10.times.Ki) of a potent inhibitor the anisotropy of the unbound fluorescent ligand is measurably different from the bound value. Fluorescence anisotropy read in an LJL Acquest fluorescence reader. |
| Affinity data for this assay | |
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