| Assay Method Information | |
| | DELFIA Assay (Dissociation Enhanced Lanthanide Fluoro-Immuno Assay) |
| Description: | Compound was evaluated for its ability to disrupt the interaction of pepJIP1 with JNK1 by using DELFIA assay. DELFIA is a heterogeneous assay whereby a biotin-linked pepJIP1 is adsorbed onto a streptavidin-coated plate followed by incubation with GST-JNK1. Subsequently, fluorescence was measured (excitation wavelength, 340 nm; emission wavelength, 615 nm). Controls include unlabeled peptide and blanks receiving no compounds. |
| Affinity data for this assay | |
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