| Assay Method Information | |
| | Aurora B Kinase Inhibition Assay |
| Description: | In vitro kinase assay using recombinant Aurora B bound to INCENP was incubated at room temperature with substrate, and test compounds in the presence of 15 uM ATP/ [gamma-33P] ATP. 33P incorporation was measured with a Beta Counter. IC50 values were calculated using ORIGIN Scientific Graphing Analyses. |
| Affinity data for this assay | |
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