Assay Method Information | |
| Pim Kinase Assay |
Description: | Assays were conducted in 384-well v-bottom polypropylene plates. Test compound was mixed with Pim and biotinylated peptide substrate, followed by immediate initiation with [gamma-33 P]ATP. Reactions were quenched and transferred to 384-well streptavidin-coated plates. After incubation, radioactivity was counted on a TopCount Scintillation Plate Reader (Packard). |
Affinity data for this assay | |
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