| Assay Method Information | |
| | FAAH Competitive ABPP Using FP-Rhodamine |
| Description: | Mouse brain membrane proteomes were preincubated with varying concentrations of inhibitors for 10 min prior to the addition of a rhodamine-tagged fluorophosphonate (FP-rhodamine). Reactions were quenched after 10 min, subjected to SDS-PAGE, and visualized in-gel using a flatbed fluorescence scanner. Labeled proteins were quantified by measuring integrated band intensities. Dose response curves obtained from three trials at each inhibitor concentration were fit using Prism software (GraphPad) to |
| Affinity data for this assay | |
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