Assay Method Information

Assay Name:  CDK Inhibition Assay
Description:  In vitro kinase assay using purified enzyme, was incubated at 30 °C with substrate, and test compounds in the presence of 15 uM ATP/ [gamma-33P] ATP. 33P incorporation was measured with a scintillation counter. IC50 values were calculated from dose-response curves.
Affinity data for this assay
 

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