| Assay Method Information | |
| | QR2 Assay and IC50 Value Determination |
| Description: | The activity of QR2 under steady-state conditions was evaluated on SpectraMax Plus 384 UV/vis spectrophotometer by monitoring the increase in absorbance of the product formazan at 610 nm which is produced from MTT. IC50 values were determined in 96-well plates. Each assay mixture contained QR2, NMeH, and MTT in a reaction buffer containing test compounds. Inhibitor concentrations ranged from 0.4 to 500 uM, and 11 different inhibitor concentrations were chosen for each IC50 value determination. Assays at each inhibitor concentration were performed in triplicate, and the average rate values and standard deviation at each rate were used to determine the IC50 values. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |