| Assay Method Information | |
| | Cdk4 and 6 Enzymatic Inhibition Assay |
| Description: | For the Ki determination assay, 200 μM stock solutions of compounds were subjected to a serial, semi-logarithmic dilution using 100% DMSO as a solvent. 10 distinct concentrations were prepared, with a dilution endpoint of 6×10−9 M in 100% DMSO. 100% DMSO was used as a control. 10 μL from each of the serial dilutions were aliquoted into separate wells of a 96-well plate and 90 μL of water were added to each of those wells. The plate was shaken thoroughly, and 5 μL from each of the plate's wells were transferred into wells of the assay plate. The final volume of wells in the assay plate was 50 μL. A11 compounds were tested at 10 assay concentrations in the range from 2×10−6 M to 6×10−11 M. The final DMSO concentration in the wells of the assay plate was 1% in all cases. |
| Affinity data for this assay | |
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