| Assay Method Information | |
| | Homogeneous Time-resolved Fluorescence Assay |
| Description: | Biochemical IC50s for JNK were determined using HTRF. In each assay the phosphor-Thr71-ATF-2 product was detected using a Europium-cryptate labeled anti-phospho-Thr71-ATF-2 antibody. Streptavidin-allophycocyanin-XL was used as the acceptor. A 10-point dose-response curve for each compound was generated in duplicate and data was fit to a four parameter logistic. |
| Affinity data for this assay | |
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