Assay Method Information | |
| Protease Inhibition Assay |
Description: | Inhibition constants were determined by a fluorometric assay with the fluorogenic substrate Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Lys(DABCYL)-Arg. The data were analyzed with the mathematical model for tight-binding inhibitors in which the concentration of inhibitor is less than or equal to the enzyme concentration. The data were fitted by nonlinear regression analysis to the equation with program Enzfitter (version 1.05). |
Affinity data for this assay | |
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