Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  Inhibition constants were determined by a fluorometric assay with the fluorogenic substrate Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Lys(DABCYL)-Arg. The data were analyzed with the mathematical model for tight-binding inhibitors in which the concentration of inhibitor is less than or equal to the enzyme concentration. The data were fitted by nonlinear regression analysis to the equation with program Enzfitter (version 1.05).
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail