| Assay Method Information | |
| | Fluorescence-Based RNase H Assay |
| Description: | Enzyme activity was measuring substrate hydrolysis of RNase H. The intact substrate has a low background fluorescent signal and provides up to 50-fold fluorescent signal enhancement following hydrolysis. RNA/DNA hybrid substrate was added to microplate wells contained test compound in reaction buffer. Reactions were started by the addition of enzyme to each well of the microplate. Samples were mixed and the plates were incubated at room temperature for 30 min. The reactions were quenched by the addition of 0.5 M EDTA. Fluorescence intensity in each well was assessed using an excitation wavelength of 490 nm and an emission wavelength of 528 nm, with cutoff filter set to 515 nm. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |