| Assay Method Information | |
| | Glycogen Phosphorylase Enzyme Assay |
| Description: | The enzymatic inhibition of phosphorylase activity was monitored using microplate reader (Bio-Rad). In brief, GPa activity was measured in the direction of glycogen synthesis by the release of phosphate from glucose-1-phosphate. Each compound was dissolved in DMSO and diluted at different concentrations for IC50 determination. The enzyme was added into the buffer containing compound, glucose-1-phosphate, and 1 mg/mL glycogen in 96-well microplates (costar). After the addition of ammonium molybdate and malachite green, reactions were run at 22 deg C for 25 min. And then the phosphate absorbance was measured at 655 nm. The IC50 values were estimated by fitting the inhibition data to a dose-dependent curve using a logistic derivative equation. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |