| Assay Method Information | |
| | BAP1 Enzyme inhibitors Dose Response Confirmation |
| Description: | NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Keith D. Wilkinson, Emory University MLSCN Grant: 1 R03 MH076382-01 Assay Overview: BAP1 (BRCA1 associated protein 1) is a member of the Ubiquitin carboxy-terminal hydrolase (UCH) family of deubiquitinating enzymes(DUB). These proteases reverse the conjugation of ubiquitin to targeted proteins. The importance of ubiquitin conjugation in many cellular processes suggests a critical role of DUBs in normal physiology and potentially in pathological conditions. In order to identify molecular probes for studying DUB function and for targeting DUB for potential therapeutic applications, an enzyme-based kinetic high throughput assay has been developed. The assay monitors the hydrolysis of simple ubiquitin conjugates such as Ub-AMC by cysteine protease activity of BAP1 and the release of free AMC. The amount of the fluorescence intensity of released free AMC is pro |
| Affinity data for this assay | |
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