| Assay Method Information | |
| | Complement C1s ELISA |
| Description: | Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 The classical pathway mediates specific antibody responses. The classical pathway is initiated by the binding of antibodies to cell surface antigens. Subsequent binding of the antibody to complement C1q subunits of C1 result in catalytically active C1s subunits. The two activated C1s subunits are then able to catalyze the assembly of the C3 convertase (complement C4b2a) from complements C2 and C4.(Ref. 1) Assay The high-throughput screen on mixture plates for complement factor C1s inhibitors and single compound IC50 determination of active compounds has been reported earlier (AID 538 and AID 787). In this assay, we use ELISA, which can determine the activity of the classical pathway as a whole, to test the activity of hits from the C1s mixture screen. Materials Human Serum was purchased from Comp |
| Affinity data for this assay | |
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