| Assay Method Information | |
| | YopH - IC50 determinations (10 point dose range) |
| Description: | Yersinia pestis is the causal agent of the bubonic plague and, although modern antibiotics are extremely effective against the malady, the plague remains a threat in many areas in the world. Outbreaks of hundreds of cases still occur in Asia, Africa and South America and, in the United States cases are reported sporadically, mainly because of people handling infected animals or by being bitten by infected wild rodent fleas (http://www.cdc.gov). YopH (Yersinia outer protein H) is a protein essential for the virulence of Yersinia. YopH is a protein tyrosine phosphatase that antagonizes several signaling pathways including those associated with the phagocytosis of bacteria by host cells (Fallman et al., 1997). Upon infection, YopH is translocated into the target cell, where it localizes to focal complexes. Several proteins have been identified as substrates for YopH including Cas, paxillin, SKAP-HOM, Fyb and Pyk (Viboud and Bliska, 2005). Because the phosphatase activity of the Yersinia |
| Affinity data for this assay | |
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