| Assay Method Information | |
| | In Vitro Assay of Compounds of the Present Invention for Inhibiting MYT1 Kinase Activity |
| Description: | Recombinant MYT1 protein and the compounds were pre-incubated at room temperature for 15 min, and 10 μM ATP was then added to start the reaction. The mixture was incubated at room temperature for another 60 min, and the ADP-GLO reagent from Promega was then added. The resulting mixture was incubated in the dark for 40 min, a detection solution was then added, and the mixture was incubated for another 45-60 min. The chemiluminescence was determined using ENVISION. The inhibition rate and IC50 were calculated compared to the DMSO group. |
| Affinity data for this assay | |
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