| Assay Method Information | |
| | Binding affinities for Adenosine receptors |
| Description: | The assay was performed as described in Chinese hamster ovary (CHO)cells stably expressing either the recombinant hA1 or hA3AR andhuman embryonic kidney (HEK) 293 cells stably expressing thehuman (h) A2AAR were cultured in DMEM and F12 (1:1), supplementedwith 10% fetal bovine serum, 100 units/mL penicillin, 100 μg/mLstreptomycin, and 2 μmol/mL glutamine. In addition, 800 μg/mLGeneticin and 500 μg/mL hygromycin were added to the A2A mediaand to the A1 and A3 media, respectively. After harvesting thecells, they were homogenized for 10 sec with an electric homogenizer,pipetted into 1-mL vials, and then stored at −80° C. until bindingexperiments were conducted. The concentration of protein wasdetermined using a BCA Protein Assay Kit from PierceBio-technology (Rockford, III.). |
| Affinity data for this assay | |
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