Assay Method Information | |
| Fluorescent Competition Assay |
Description: | The binding affinities to FtsZ were determined by a fluorescent competition assay with 2'/3'-O-(N-methyl-anthraniloyl)-guanosine-5'-triphosphate (mantGTP). Polyermization studies at 90 degree angle light scattering was conducted. Where both wavelength, excitation and emission were set at 350 nM and slit widths were 1.5 nM |
Affinity data for this assay | |
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