Assay Method Information

Assay Name:  Inhibition Assay
Description:  IP3K reactions were carried out in a 100ul solution that contained Tris-Cl, EGTA, ATP, DTT, 2,3-diphosphoglycerate, D-l(1,4,5)P3, [3H]-l(1,4,5)P3 and various amounts of synthetic purine analogues.
Affinity data for this assay
 

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