Assay Method Information | |
| ChEMBL_1833218 (CHEMBL4333226) |
Description: | Inhibition of PFKFB3 in human HCT116 cell lysates assessed as reduction in glycolytic-flux induced fructose-2,6-bisphosphate formation using fructose-6-phosphate as substrate treated with cells prior to glucose stimulation followed by PFK1 and substrate addition and measured at 0.5 mins interval for 45 mins |
Affinity data for this assay | |
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