| Assay Method Information | |
| | ChEMBL_2186512 (CHEMBL5098594) |
| Description: | Inhibition of N-terminal his6-tagged wild type recombinant human SHP2 (1 to 597 residues) expressed in Escherichia coli BL21 (DE3) cells using fluorogenic 6,8-difluoro-4-methylumbelliferyl phosphate as substrate pretreated for 30 mins followed by substrate addition and measured after 1 hr by fluorescence assay |
| Affinity data for this assay | |
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