Assay Method Information | |
| Enzyme Inhibition Assay |
Description: | The phosphorylation of L-Hse was monitored by coupling the formation of ADP with pyruvate kinase and lactate dehydrogenase (PK/LDH). The resulting oxidation of NADH was monitored at 340 nm by using a SpectraMax plate reader in a 96-well format. |
Affinity data for this assay | |
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