| Assay Method Information | |
| | MAO Enzyme Assays |
| Description: | The buffer system used was a 0.1 M phosphate buffer (pH 7.4). The assays were carried out in a fluorescent 96-well plate. The final concentrations of MAO-A and MAO-B were 6 µg/mL and 15 µg/mL, respectively. The final concentration of kynuramine was 40 µM for MAO-A or 20 µM for MAO-B. The compounds (10 mM stock solution in DMSO; less than 2% (v/v) final concentration of DMSO in the assay) were incubated with MAO and the substrate for 20 min, and quenched with the addition of 2 N NaOH. |
| Affinity data for this assay | |
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