| Assay Method Information | |
| | DXR Inhibition Assay |
| Description: | H-DXR was pre-incubated during 2 min in the presence of the inhibitors at different concentrations and DXP (480 然). NADPH (160 然 final concentration) was then added to measure the residual activity. The flavonoids are poorly soluble in water. Flavonoids solutions at different concentrations were prepared in DMSO by serial dilutions from 100 mM stock solutions. Aliquots of the solutions were added in the assays so that the concentration of DMSO did not exceed 0.4% (v/v). The inhibition mechanism of myricetin was studied by preincubating H-DXR with DXP at varied concentrations (96 480 然) and fixed concentrations of myricetin (0.5, 1 and 2 然). The enzymatic reaction was initiated by NADPH (final concentration160 然). Fosmidomycin was used as a positive reference. |
| Affinity data for this assay | |
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